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PPP2R3A Rabbit pAb

Anti-PPP2R3A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220830

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220830
Product Name:   Anti-PPP2R3A Rabbit Polyclonal Antibody
Synonyms:   PR72; PR130; PPP2R3
UNIPROT ID:   Q06190 (Gene Accession – NP_001177376 )
Background:    This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B’/PR61, and B’/PR72 families.
Immunogen:    Synthetic peptide of human PPP2R3A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 220830(PPP2R3A Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 220830(Anti-PPP2R3A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 220830(Anti-PPP2R3A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D262078(Anti-PPP2R3A Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T cells, PC3 cells;
Primary antibody: 220830(PPP2R3A Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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