NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: N263506 |
Product Name: Anti-PRAS40 Rabbit Monoclonal Antibody |
Synonyms: AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate |
UNIPROT ID: Q96B36 |
Background: PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183. |
Immunogen: A synthetic peptide of human PRAS40 |
Applications: WB,IP |
Recommended Dilutions: WB: 1/500-1/1000 IP: 1/20 |
Host Species: Rabbit |
Clonality: Rabbit Monoclonal |
Clone ID: R05-3A5 |
MW: Calculated MW: 27 kDa; Observed MW: 40 kDa |
Isotype: IgG |
Purification: Affinity Purified |
Species Reactivity: Human,Rat |
Conjugation: Unconjugated |
Modification: Unmodified |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: Cell Biology |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of PRAS40 in Hela, A549, C6 lysates using PRAS40 antibody. |