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PRKG2 Rabbit pAb

Anti-PRKG2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220086

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220086
Product Name:   Anti-PRKG2 Rabbit Polyclonal Antibody
Synonyms:   cGKII; PRKGR2
UNIPROT ID:   Q13237 (Gene Accession – NP_006250 )
Background:    cGKII (cGMP-dependent protein kinase type II) is a major receptor of intracellular cGMP and mediates a plethora of physiological responses. cGKII contains a conserved leucine zipper motif at the amino-terminus. It is expressed in small intestine, colon, prostate, and human brain tissues, and the cGKII gene maps to chromosome 4q13.1-q21.1. cGKII has been shown to regulate the ion transport system in the intestine. Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains. The translocation of cGKII from the cytosol to the membrane allows it to function properly in regulating intestinal ion transport.
Immunogen:    Synthetic peptide of human PRKG2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220086(PRKG2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220086(Anti-PRKG2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 220086(Anti-PRKG2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260929(Anti-PRKG2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 50 μg;
Lane 1-2: Mouse brain tissue, Mouse lung tissue;
Primary antibody: 220086(PRKG2 Antibody) at dilution 1/160;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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