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PRMT5 Rabbit pAb

Anti-PRMT5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213719

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213719
Product Name:   Anti-PRMT5 Rabbit Polyclonal Antibody
Synonyms:   JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
UNIPROT ID:   O14744 (Gene Accession – NP_001034708 )
Background:    Protein arginine methyltransferase 5 has been shown to interact with WD repeat-containing protein 77, CLNS1A, Janus kinase 2, SNRPD3 and SUPT5H.Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation.
Immunogen:    Synthetic peptide of human PRMT5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 213719(PRMT5 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 213719(Anti-PRMT5 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 213719(Anti-PRMT5 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D160716(Anti-PRMT5 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562 cells, hela cells, 293T cells;
Primary antibody: 213719(PRMT5 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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