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PRPF8 Rabbit pAb

Anti-PRPF8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221811

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221811
Product Name:   Anti-PRPF8 Rabbit Polyclonal Antibody
Synonyms:   PRP8; RP13; HPRP8; PRPC8; SNRNP220
UNIPROT ID:   Q6P2Q9 (Gene Accession – NP_006436 )
Background:    Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Immunogen:    Synthetic peptide of human PRPF8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 221811(PRPF8 Antibody) at a dilution of 1/20(Nucleus or Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 221811(Anti-PRPF8 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 221811(Anti-PRPF8 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D263565(Anti-PRPF8 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T, K562, Raji, Hela and NIH/3T3 cell lysates;
Primary antibody: 221811(PRPF8 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 50 seconds
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