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PSMB2 Rabbit pAb

Anti-PSMB2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219007

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219007
Product Name:   Anti-PSMB2 Rabbit Polyclonal Antibody
Synonyms:   HC7-I
UNIPROT ID:   P49721 (Gene Accession – BC000268 )
Background:    The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen:    Fusion protein of human PSMB2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 219007(PSMB2 Antibody) at a dilution of 1/130(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 219007(Anti-PSMB2 Antibody) at dilution 1/130.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219007(Anti-PSMB2 Antibody) at a dilution of 1/130.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225640(Anti-PSMB2 Antibody) at dilution 1/130.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T, Jurkat, HT29, K562, LOVO, Hela and HepG2 cell lysates;
Primary antibody: 219007(PSMB2 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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