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PSMB8 Rabbit pAb

Anti-PSMB8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220678

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220678
Product Name:   Anti-PSMB8 Rabbit Polyclonal Antibody
Synonyms:   JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
UNIPROT ID:   P28062 (Gene Accession – NP_004150 )
Background:    The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
Immunogen:    Synthetic peptide of human PSMB8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220678(PSMB8 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220678(Anti-PSMB8 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 220678(Anti-PSMB8 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D261863(Anti-PSMB8 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: 231 cells, A431 cells, Mouse liver tissue, Human placenta tissue, Jurkat cells, Raji cells;
Primary antibody: 220678(PSMB8 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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