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PTP4A2 Rabbit pAb

Anti-PTP4A2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216748

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216748
Product Name:   Anti-PTP4A2 Rabbit Polyclonal Antibody
Synonyms:   HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
UNIPROT ID:   Q12974 (Gene Accession – BC070182 )
Background:    The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11, 12 and 17.
Immunogen:    Fusion protein of human PTP4A2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216748(PTP4A2 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216748(Anti-PTP4A2 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 216748(Anti-PTP4A2 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D221162(Anti-PTP4A2 Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane 1-3: Hela cells, Jurkat cells, SKOV3 cells;
Primary antibody: 216748(PTP4A2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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