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PTRH2 Rabbit pAb

Anti-PTRH2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217724

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217724
Product Name:   Anti-PTRH2 Rabbit Polyclonal Antibody
Synonyms:   BIT1; PTH2; CFAP37; CGI-147
UNIPROT ID:   Q9Y3E5 (Gene Accession – BC006807 )
Background:    PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis.
Immunogen:    Fusion protein of human PTRH2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217724(PTRH2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217724(Anti-PTRH2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 217724(Anti-PTRH2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D222953(Anti-PTRH2 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: K562 cells, hepg2 cells, 231 cells, Jurkat cells, Raji cells;
Primary antibody: 217724(PTRH2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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