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RAB3A Rabbit pAb

Anti-RAB3A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217729

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217729
Product Name:   Anti-RAB3A Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P20336 (Gene Accession – BC011782 )
Background:    Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity). Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity). Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity). Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity). Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790). Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141). Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity).
Immunogen:    Fusion protein of human RAB3A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 217729(RAB3A Antibody) at a dilution of 1/110(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 217729(Anti-RAB3A Antibody) at dilution 1/110.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217729(Anti-RAB3A Antibody) at a dilution of 1/110.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222958(Anti-RAB3A Antibody) at dilution 1/110.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human cerebella tissue, Human cerebrum tissue and Mouse brain tissue lysates;
Primary antibody: 217729(RAB3A Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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