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Rad23B (5H1) Mouse mAb

Anti-Rad23B (5H1) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261115

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261115
Product Name:   Anti-Rad23B (5H1) Mouse Monoclonal Antibody
Synonyms:   RAD23B; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58
UNIPROT ID:   P54727
Background:    The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen:    Purified recombinant human hHR23b protein fragments expressed in E.coli.
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5H1-A10-A7
MW:    Calculated MW: 43 kDa; Observed MW: 58 kDa
Isotype:    IgG2b
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat,Monkey,Hamster
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Rad23B (5H1) in HeLa using hHR23b antibody.

Western blot analysis of hHR23b in A431, K562, Jurkat, C6, 3T3 and Hela lysates using hHR23b antibody.

Immunohistochemistry analysis of paraffin-embedded Prostate Cancer using hHR23b antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Rad23B (5H1) in K562, SW480, CHO-K1, 3T3 and COS7 lysates using Rad23B (5H1) antibody
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