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RAD51 Rabbit pAb

Anti-RAD51 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220843

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220843
Product Name:   Anti-RAD51 Rabbit Polyclonal Antibody
Synonyms:   RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
UNIPROT ID:   Q06609 (Gene Accession – NP_001157741 )
Background:    The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage.
Immunogen:    Synthetic peptide of human RAD51
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220843(RAD51 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220843(Anti-RAD51 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 220843(Anti-RAD51 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D262104(Anti-RAD51 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cells;
Primary antibody: 220843(RAD51 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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