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Raf1 Rabbit pAb

Anti-Raf1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225480

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225480
Product Name:   Anti-Raf1 Rabbit pAb
Synonyms:   RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
UNIPROT ID:   P04049
Background:    Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Immunogen:    The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300
Applications:    ICC/IF,WB,IHC-F,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 73 kDa; Observed MW: 73 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.

Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).
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