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RAP1A Rabbit pAb

Anti-RAP1A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216352

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216352
Product Name:   Anti-RAP1A Rabbit Polyclonal Antibody
Synonyms:   RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
UNIPROT ID:   P62834 (Gene Accession – BC014086 )
Background:    This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 216352(RAP1A Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 216352(Anti-RAP1A Antibody) at dilution 1/45.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 216352(Anti-RAP1A Antibody) at a dilution of 1/45.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D220263(Anti-RAP1A Antibody) at dilution 1/45.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue and A431 cell;
Primary antibody: 216352(RAP1A Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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