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RASGRP3 Rabbit pAb

Anti-RASGRP3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219520

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219520
Product Name:   Anti-RASGRP3 Rabbit Polyclonal Antibody
Synonyms:   GRP3
UNIPROT ID:   Q8IV61 (Gene Accession – BC027849 )
Background:    The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers.
Immunogen:    Fusion protein of human RASGRP3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219520(RASGRP3 Antibody) at a dilution of 1/85(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219520(Anti-RASGRP3 Antibody) at dilution 1/85.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 219520(Anti-RASGRP3 Antibody) at a dilution of 1/85.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D227459(Anti-RASGRP3 Antibody) at dilution 1/85.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 231 cell lysate;
Primary antibody: 219520(RASGRP3 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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