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RBM5 Rabbit pAb

Anti-RBM5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217746

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217746
Product Name:   Anti-RBM5 Rabbit Polyclonal Antibody
Synonyms:   G15; H37; RMB5; LUCA15
UNIPROT ID:   P52756 (Gene Accession – BC002957 )
Background:    This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer.
Immunogen:    Fusion protein of human RBM5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 217746(RBM5 Antibody) at a dilution of 1/30(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 217746(Anti-RBM5 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217746(Anti-RBM5 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D222993(Anti-RBM5 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: 823 cells, A549 cells, K562 cells, 293T cells, hepg2 cells, huvec cells;
Primary antibody: 217746(RBM5 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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