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RGS14 Rabbit pAb

Anti-RGS14 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S211491

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S211491
Product Name:   Anti-RGS14 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   O43566 (Gene Accession – BC014094 )
Background:    This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Immunogen:    Fusion protein of human RGS14
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 211491(RGS14 Antibody) at a dilution of 1/160(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 211491(Anti-RGS14 Antibody) at dilution 1/160.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 211491(RGS14 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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