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RHEB Rabbit pAb

Anti-RHEB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220748

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220748
Product Name:   Anti-RHEB Rabbit Polyclonal Antibody
Synonyms:   RHEB2
UNIPROT ID:   Q15382 (Gene Accession – NP_005605 )
Background:    This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
Immunogen:    Synthetic peptide of human RHEB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220748(RHEB Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220748(Anti-RHEB Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 220748(Anti-RHEB Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D261955(Anti-RHEB Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Human brain malignant glioma tissue, Jurkat cells, Raji cells, 231 cells, human fetal brain tissue, Hela cells;
Primary antibody: 220748(RHEB Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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