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RhoA/B/C Rabbit pAb

Anti-RhoA/B/C Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225549

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225549
Product Name:   Anti-RhoA/B/C Rabbit pAb
Synonyms:   ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
UNIPROT ID:   P61586/P62745/P08134
Background:    RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
Immunogen:    A synthesized peptide derived from human Rho A + B + C
Applications:    WB,IHC-P,ICC/IF,FC
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 22 kDa; Observed MW: 22 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Rho A + B + C expression in (1) Jurkat lysates; (2) NIH/3T3 lysates using RhoA/B/C antibody

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma using Rho A + B + C antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Rho A + B + C in SH-SY5Y and rat Brain lysates using Rho A + B + C antibody.

Immunofluorescent analysis of RhoA/B/C in Hela using RhoA/B/C antibody.
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