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RHOF Rabbit pAb

Anti-RHOF Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219549

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219549
Product Name:   Anti-RHOF Rabbit Polyclonal Antibody
Synonyms:   RIF; ARHF
UNIPROT ID:   Q9HBH0 (Gene Accession – BC018208 )
Background:    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Immunogen:    Fusion protein of human RHOF
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219549(RHOF Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219549(Anti-RHOF Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: RAW264.7 cell lysate;
Primary antibody: 219549(RHOF Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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