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RIPK1 Rabbit pAb

Anti-RIPK1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217764

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217764
Product Name:   Anti-RIPK1 Rabbit Polyclonal Antibody
Synonyms:   RIP; RIP1; AIEFL; IMD57; RIP-1
UNIPROT ID:   Q13546 (Gene Accession – BC126254 )
Background:    This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.
Immunogen:    Fusion protein of human RIPK1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217764(RIPK1 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217764(Anti-RIPK1 Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549 cell, MCF7 cell, Mouse kidney tissue lysates;
Primary antibody: 217764(RIPK1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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