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RNF40 Rabbit pAb

Anti-RNF40 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217772

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217772
Product Name:   Anti-RNF40 Rabbit Polyclonal Antibody
Synonyms:   BRE1B; RBP95; STARING
UNIPROT ID:   O75150 (Gene Accession – BC006133 )
Background:    The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.
Immunogen:    Fusion protein of human RNF40
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217772(RNF40 Antibody) at a dilution of 1/25(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217772(Anti-RNF40 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217772(Anti-RNF40 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D223043(Anti-RNF40 Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela cells, 231 cells, A172 cells;
Primary antibody: 217772(RNF40 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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