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ROCK2 Rabbit pAb

Anti-ROCK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216766

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216766
Product Name:   Anti-ROCK2 Rabbit Polyclonal Antibody
Synonyms:   ROCK-II
UNIPROT ID:   O75116 (Gene Accession – BC111801 )
Background:    The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Immunogen:    Fusion protein of human ROCK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216766(ROCK2 Antibody) at a dilution of 1/130(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216766(Anti-ROCK2 Antibody) at dilution 1/130.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse brain tissue lysate;
Primary antibody: 216766(ROCK2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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