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RPL14 Rabbit pAb

Anti-RPL14 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221802

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221802
Product Name:   Anti-RPL14 Rabbit Polyclonal Antibody
Synonyms:   L14; RL14; hRL14; CTG-B33; CAG-ISL-7
UNIPROT ID:   P50914 (Gene Accession – NP_003964 )
Background:    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5′-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Immunogen:    Synthetic peptide of human RPL14
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221802(RPL14 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221802(Anti-RPL14 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: A431, HEPG2, 231, Jurkat, Hela and 293T cell lysates;
Primary antibody: 221802(RPL14 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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