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RPL3 Rabbit pAb

Anti-RPL3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218684

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218684
Product Name:   Anti-RPL3 Rabbit Polyclonal Antibody
Synonyms:   L3; ASC-1; TARBP-B
UNIPROT ID:   P39023 (Gene Accession – BC002408 )
Background:    Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene’s introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Immunogen:    Fusion protein of human RPL3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218684(RPL3 Antibody) at a dilution of 1/20(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218684(Anti-RPL3 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 218684(Anti-RPL3 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D224939(Anti-RPL3 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, Jurkat and Raji cell lysates;
Primary antibody: 218684(RPL3 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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