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RPS27A Rabbit pAb

Anti-RPS27A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218977

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218977
Product Name:   Anti-RPS27A Rabbit Polyclonal Antibody
Synonyms:   UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
UNIPROT ID:   P62979 (Gene Accession – BC001392 )
Background:    Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
Immunogen:    Fusion protein of human RPS27A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 218977(RPS27A Antibody) at a dilution of 1/40(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 218977(Anti-RPS27A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 218977(Anti-RPS27A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D225594(Anti-RPS27A Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HL60 cell, Mouse kidney tissue lysates;
Primary antibody: 218977(RPS27A Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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