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RPS28 Rabbit pAb

Anti-RPS28 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215876

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215876
Product Name:   Anti-RPS28 Rabbit Polyclonal Antibody
Synonyms:   S28; DBA15
UNIPROT ID:   P62857 (Gene Accession – NP_001022 )
Background:    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Immunogen:    Synthetic peptide of human RPS28
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 215876(RPS28 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 215876(Anti-RPS28 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 215876(Anti-RPS28 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D164039(Anti-RPS28 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: 293T and PC3 cell, Mouse spleen tissue, 231 cell, Human breast cancer tissue, Mouse kidney tissue lysates;
Primary antibody: 215876(RPS28 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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