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RPS6KB2 Rabbit pAb

Anti-RPS6KB2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220777

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220777
Product Name:   Anti-RPS6KB2 Rabbit Polyclonal Antibody
Synonyms:   KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
UNIPROT ID:   Q9UBS0 (Gene Accession – NP_003943 )
Background:    This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
Immunogen:    Synthetic peptide of human RPS6KB2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 220777(RPS6KB2 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 220777(Anti-RPS6KB2 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, K562 cells;
Primary antibody: 220777(RPS6KB2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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