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RRAGA Rabbit pAb

Anti-RRAGA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217786

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217786
Product Name:   Anti-RRAGA Rabbit Polyclonal Antibody
Synonyms:   FIP1; RAGA; FIP-1
UNIPROT ID:   Q7L523 (Gene Accession – BC006433 )
Background:    Ras-related GTP-binding protein A is a protein that in humans is encoded by the RRAGAgene. Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.
Immunogen:    Fusion protein of human RRAGA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217786(RRAGA Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217786(Anti-RRAGA Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hepg2 cells, hela cells;
Primary antibody: 217786(RRAGA Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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