Email: info@neweastbio.com   |  Call: 6109452007

RRP8 Rabbit pAb

Anti-RRP8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219130

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219130
Product Name:   Anti-RRP8 Rabbit Polyclonal Antibody
Synonyms:   NML; KIAA0409
UNIPROT ID:   O43159 (Gene Accession – BC001071 )
Background:    Essential component of the eNoSC (energy-dependent nucleolar silencing) comple,x a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at ‘Lys-9’ (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the comple,x RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Immunogen:    Fusion protein of human RRP8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219130(RRP8 Antibody) at a dilution of 1/80(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219130(Anti-RRP8 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219130(Anti-RRP8 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225896(Anti-RRP8 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human cerebella tissue, K562 cell lysates;
Primary antibody: 219130(RRP8 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
          Publications