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SAFB1 (2E8) Mouse mAb

Anti-SAFB1 (2E8) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261053

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261053
Product Name:   Anti-SAFB1 (2E8) Mouse Monoclonal Antibody
Synonyms:   SAFB; HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein
UNIPROT ID:   Q15424
Background:    This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a ‘transcriptosome complex’ in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Purified recombinant human SAFB1 protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,ChIP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 ChIP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    2E8-E2-G6
MW:    Calculated MW: 103 kDa; Observed MW: 130 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of SAFB1 (2E8) in HeLa using SAFB1 antibody.

Western blot analysis of SAFB1 in A431, MDA-MB-468, Breast cancer, ZR751 and MCF-7 lysates using SAFB1 antibody.
          Publications