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SETD3 Rabbit pAb

Anti-SETD3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218174

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218174
Product Name:   Anti-SETD3 Rabbit Polyclonal Antibody
Synonyms:   hSETD3; C14orf154
UNIPROT ID:   Q86TU7 (Gene Accession – BC009054 )
Background:    Protein-histidine N-methyltransferase that specifically mediates methylation of actin at ‘His-73’. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Immunogen:    Fusion protein of human SETD3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218174(SETD3 Antibody) at a dilution of 1/140(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218174(Anti-SETD3 Antibody) at dilution 1/140.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218174(Anti-SETD3 Antibody) at a dilution of 1/140.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223876(Anti-SETD3 Antibody) at dilution 1/140.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human metastatic malignant melanoma tissue lysate;
Primary antibody: 218174(SETD3 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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