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SH3BGR Rabbit pAb

Anti-SH3BGR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219278

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219278
Product Name:   Anti-SH3BGR Rabbit Polyclonal Antibody
Synonyms:   21-GARP
UNIPROT ID:   P55822 (Gene Accession – BC006371 )
Background:    SH3 domain binding glutamic acid-rich protein (SH3BGR), also designated 21-glutamic acid-rich protein (21-GARP), is a 239-amino acid protein differentially expressed in heart and skeletal muscle. Its proline-rich region contains the consensus sequence for an SH3-binding domain and its acidic C-terminal region contains a glutamic acid-rich domain which may assume a coiled-coil structure. SH3BGR may be part of a multimeric comple,x as it contains 2 functional domains involved in protein-protein interactions. The SH3BGR gene maps proximal to HMG14 on chromosome 21q22.3.
Immunogen:    Fusion protein of human SH3BGR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219278(SH3BGR Antibody) at a dilution of 1/60(Cytoplasm, Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 219278(Anti-SH3BGR Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human muscle tissue lysate;
Primary antibody: 219278(SH3BGR Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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