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SIGLEC12 Rabbit pAb

Anti-SIGLEC12 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217816

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217816
Product Name:   Anti-SIGLEC12 Rabbit Polyclonal Antibody
Synonyms:   S2V; SLG; SIGLECL1; Siglec-XII
UNIPROT ID:   Q96PQ1 (Gene Accession – BC035809 )
Background:    Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like.
Immunogen:    Fusion protein of human SIGLEC12
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217816(SIGLEC12 Antibody) at a dilution of 1/30(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217816(Anti-SIGLEC12 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse bladder tissue;
Primary antibody: 217816(SIGLEC12 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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