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SIGLEC5 Rabbit pAb

Anti-SIGLEC5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217256

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217256
Product Name:   Anti-SIGLEC5 Rabbit Polyclonal Antibody
Synonyms:   CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
UNIPROT ID:   O15389 (Gene Accession – BC029896 )
Background:    This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
Immunogen:    Fusion protein of human SIGLEC5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217256(SIGLEC5 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217256(Anti-SIGLEC5 Antibody) at dilution 1/55.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Jurkat cell, Mouse liver tissue, LO2 cell lysates;
Primary antibody: 217256(SIGLEC5 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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