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SIGLEC7 Rabbit pAb

Anti-SIGLEC7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214216

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214216
Product Name:   Anti-SIGLEC7 Rabbit Polyclonal Antibody
Synonyms:   p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
UNIPROT ID:   Q9Y286 (Gene Accession – NP_055200 )
Background:    Sialic acid-binding Ig-like lectin 7 is a protein that in humans is encoded by the SIGLEC7 gene. SIGLEC7 has also been designated as CD328 (cluster of differentiation 328). Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Immunogen:    Synthetic peptide of human SIGLEC7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience, Cardiovascular, Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 214216(SIGLEC7 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 214216(Anti-SIGLEC7 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 214216(Anti-SIGLEC7 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D161525(Anti-SIGLEC7 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cells;
Primary antibody: 214216(SIGLEC7 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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