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SKIL Rabbit pAb

Anti-SKIL Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S211580

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S211580
Product Name:   Anti-SKIL Rabbit Polyclonal Antibody
Synonyms:   SNO; SnoA; SnoI; SnoN
UNIPROT ID:   P12757 (Gene Accession – BC059386 )
Background:    The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.
Immunogen:    Fusion protein of human SKIL
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 211580(SKIL Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 211580(Anti-SKIL Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 211580(Anti-SKIL Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D123196(Anti-SKIL Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Huvec cells;
Primary antibody: 211580(SKIL Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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