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SLC1A6 Rabbit pAb

Anti-SLC1A6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219753

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219753
Product Name:   Anti-SLC1A6 Rabbit Polyclonal Antibody
Synonyms:   EAAT4
UNIPROT ID:   P48664 (Gene Accession – NP_005062 )
Background:    Excitatory Amino Acid Transporters (EAATs) are membrane-bound proteins that are localized in glial cells and pre-synaptic glutamatergic nerve endings. EAATs transport the excitatory neurotransmitters L-glutamate and D-aspartate, a process that is essential for terminating the postsynaptic action of glutamate. The re-uptake of amino acid neurotransmitters by EAAT proteins has been shown to protect neurons from excitotoxicity, which is caused by the accumulation of amino acid neurotransmitters. EAAT4 is an aspartate/glutamate transporter that is expressed predominantly in the cerebellum. The transport activity encoded by EAAT4 has high apparent affinity for L-aspartate and L-glutamate, and has a pharmacologic profile consistent with previously described cerebellar transport activities. EAAT5 is a glutamate transporter coupled to a chloride conductance which is expressed primarily in retina. Although EAAT5 shares the structural homologies of the EAAT family, a novel feature of the EAAT5 sequence is a carboxy-terminal motif previously identified in N-ethyl-D-aspartate receptors and potassium channels and shown to confer interactions with a family of synaptic proteins that promote ion channel clustering.
Immunogen:    Synthetic peptide of human SLC1A6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219753(SLC1A6 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219753(Anti-SLC1A6 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 219753(Anti-SLC1A6 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260282(Anti-SLC1A6 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human testis tissue, A375 cells;
Primary antibody: 219753(SLC1A6 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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