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SLC7A11 Rabbit pAb

Anti-SLC7A11 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221146

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221146
Product Name:   Anti-SLC7A11 Rabbit Polyclonal Antibody
Synonyms:   xCT; CCBR1
UNIPROT ID:   Q9UPY5 (Gene Accession – NP_055146 )
Background:    This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.
Immunogen:    Synthetic peptide of human SLC7A11
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 221146(SLC7A11 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 221146(Anti-SLC7A11 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 and 293T cell lysates;
Primary antibody: 221146(SLC7A11 Antibody) at dilution 1/1600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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