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Smad3 Rabbit mAb

Anti-Smad3 Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N261657

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261657
Product Name:   Anti-Smad3 Rabbit Monoclonal Antibody
Synonyms:   SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
UNIPROT ID:   P84022
Background:    Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Immunogen:    A synthetic peptide of human Smad3
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R09-7H4
MW:    Calculated MW: 48 kDa; Observed MW: 52 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody,and DAPI(blue).

Western blot analysis of Smad3 in Jurkat, C6, Hela lysates using Smad3 antibody.
          Publications