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SNAP25 Rabbit pAb

Anti-SNAP25 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216788

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216788
Product Name:   Anti-SNAP25 Rabbit Polyclonal Antibody
Synonyms:   RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
UNIPROT ID:   P60880 (Gene Accession – BC010647 )
Background:    Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
Immunogen:    Fusion protein of human SNAP25
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216788(SNAP25 Antibody) at a dilution of 1/25(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216788(Anti-SNAP25 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216788(Anti-SNAP25 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D221238(Anti-SNAP25 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cells;
Primary antibody: 216788(SNAP25 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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