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SPATA20 Rabbit pAb

Anti-SPATA20 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220209

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220209
Product Name:   Anti-SPATA20 Rabbit Polyclonal Antibody
Synonyms:   SSP411; Tisp78
UNIPROT ID:   Q8TB22 (Gene Accession – NP_001245301 )
Background:    The protein may play a role in fertility regulation. It were significantly upregulated in individual bile samples from Cholangiocarcinoma patients by Western blotting and Immunohistochemistry, relative to normal tissues.It acts as a potential serum diagnostic biomarker for Cholangiocarcinoma.
Immunogen:    Synthetic peptide of human SPATA20
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 220209(SPATA20 Antibody) at a dilution of 1/70(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 220209(Anti-SPATA20 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220209(Anti-SPATA20 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261141(Anti-SPATA20 Antibody) at dilution 1/70.

Gel: 10%SDS-PAGE, Lysate: 60 μg;
Lane 1-3: A549 cells, lncap cells, human seminoma tissue;
Primary antibody: 220209(SPATA20 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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