Email: info@neweastbio.com   |  Call: 6109452007

SPCS2 Rabbit pAb

Anti-SPCS2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218130

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218130
Product Name:   Anti-SPCS2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q15005 (Gene Accession – BC082231 )
Background:    SPCS2 (signal peptidase complex subunit 2), also known as SPC25 or microsomal signal peptidase 25 kDa subunit, is a 226 amino acid multi-pass membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER), and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS2 removes signal peptides from nascent proteins as they are translocated into the lumen of the ER. The gene encoding SPCS2 is located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome.
Immunogen:    Fusion protein of human SPCS2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218130(SPCS2 Antibody) at a dilution of 1/40(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218130(Anti-SPCS2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218130(Anti-SPCS2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D223784(Anti-SPCS2 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: K-562 cell, Human placenta tissue, Mouse adrenal gland tissue, HT-29 cell, NIH/3T3 cell, Rat lung tissue;
Primary antibody: 218130(SPCS2 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
          Publications