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SPDEF Rabbit pAb

Anti-SPDEF Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216722

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216722
Product Name:   Anti-SPDEF Rabbit Polyclonal Antibody
Synonyms:   PDEF; bA375E1.3
UNIPROT ID:   O95238 (Gene Accession – BC021299 )
Background:    The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human SPDEF
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 216722(SPDEF Antibody) at a dilution of 1/75(Nucleus).

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human cerebella tissue, Human cerebrum tissue, Mouse brain tissue lysates;
Primary antibody: 216722(SPDEF Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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