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SPHK2 Rabbit pAb

Anti-SPHK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217166

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217166
Product Name:   Anti-SPHK2 Rabbit Polyclonal Antibody
Synonyms:   SK 2; SK-2; SPK 2; SPK-2
UNIPROT ID:   Q9NRA0 (Gene Accession – BC006161 )
Background:    This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen:    Fusion protein of human SPHK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217166(SPHK2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217166(Anti-SPHK2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 217166(Anti-SPHK2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D221906(Anti-SPHK2 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: RAMOS cell lysate;
Primary antibody: 217166(SPHK2 Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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