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SPRY3 Rabbit pAb

Anti-SPRY3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217850

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217850
Product Name:   Anti-SPRY3 Rabbit Polyclonal Antibody
Synonyms:   spry-3
UNIPROT ID:   O43610 (Gene Accession – BC069609 )
Background:    Inhibits neurite branching, arbor length and neurite complexity (By similarity). Inhibits EGF-mediated p42/44 ERK signaling (By similarity). Negatively regulates the MAPK cascade, resulting in a reduction of extracellular matrix protein accumulation (PubMed:30878395). May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis (PubMed:9458049).
Immunogen:    Fusion protein of human SPRY3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 217850(SPRY3 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 217850(Anti-SPRY3 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 217850(Anti-SPRY3 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D223220(Anti-SPRY3 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse brain tissue lysate;
Primary antibody: 217850(SPRY3 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
          Publications