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SPRY4 Rabbit pAb

Anti-SPRY4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217849

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217849
Product Name:   Anti-SPRY4 Rabbit Polyclonal Antibody
Synonyms:   HH17
UNIPROT ID:   Q9C004 (Gene Accession – BC125095 )
Background:    This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 217849(SPRY4 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 217849(Anti-SPRY4 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 217849(Anti-SPRY4 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D223219(Anti-SPRY4 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela and Hepg2 cell, Mouse brain tissue;
Primary antibody: 217849(SPRY4 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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