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SPTA1 Rabbit pAb

Anti-SPTA1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221470

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221470
Product Name:   Anti-SPTA1 Rabbit Polyclonal Antibody
Synonyms:   EL2; HPP; HS3; SPH3; SPTA
UNIPROT ID:   P02549 (Gene Accession – NP_003117 )
Background:    Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia.
Immunogen:    Synthetic peptide of human SPTA1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 60-450;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 221470(SPTA1 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 221470(Anti-SPTA1 Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue lysate;
Primary antibody: 221470(SPTA1 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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