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SQSTM1 Rabbit pAb

Anti-SQSTM1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222050

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222050
Product Name:   Anti-SQSTM1 Rabbit Polyclonal Antibody
Synonyms:   p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
UNIPROT ID:   Q13501 (Gene Accession – NP_003891 )
Background:    This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Immunogen:    Synthetic peptide of human SQSTM1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 222050(SQSTM1 Antibody) at a dilution of 1/25(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 222050(Anti-SQSTM1 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 222050(Anti-SQSTM1 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D263941(Anti-SQSTM1 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2, Raji, Hela cell lysates;
Primary antibody: 222050(SQSTM1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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