Email: info@neweastbio.com   |  Call: 6109452007

Src Rabbit pAb

Anti-Src Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225271

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225271
Product Name:   Anti-Src Rabbit pAb
Synonyms:   SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
UNIPROT ID:   P12931
Background:    Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Immunogen:    Peptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 60 kDa; Observed MW: 60 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using Src (Ab529) antibody(left) or the same antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of Src in HT-29, Hela and MDA lysates using Src (Ab529) antibody.

Western blot analysis of Src in Hela, C6, 3T3 and PC-12 lysates using Src (Ab529) antibody.
          Publications